In article <[email protected]>, [email protected] says... > > Hi team - > > Anyone have a good protocol for isolating platelets and removing plasma > proteins through a Sepharose column? I've been having trouble with this and > fresh blood is hard to come by!!!
You can not separate cell typess by gel filtration. This is usually done by centrifugation, either fractionated or in an isotonic gradient. Such gradients can be formed for example with ficoll, metrizamide or nycodenz. Since only cells, but not the plasma proteins, enter such a gradient, this step will also separate the cells from the proteins. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
