Hello all, I need to transform S aureus strain with a plasmid of my interest.
In the biorad gene pulser protocol booklet, they mention using SMMP (sucrose, MgCl2, maleic acid and penassay broth) for reviving the s aureus after the electroporation step. I was wondering what is the role of maleic acid in this preparation. Can it be substituted with anything else? -- -- Yoginee Budhkar Contact: +91 9223355677 _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
