Hi, We use bromophenol blue to colour the stacking gel, which migrates as the dye front during the electrophoresis run. As it runs out of the gel, it doesn't really matter what concentration it's used at, but as a guide we use about 20-30ul of a 1% w/v solution per 10ml of stacking monomer.
Message: 2 Date: Tue, 18 May 2010 12:37:48 +0200 From: "Iraz Toprak Aydin" <[email protected]> Subject: how to color the stacking gel? To: <[email protected]> Message-ID: <001501caf676$2937b150$7ba713...@[email protected]> Content-Type: text/plain; charset="us-ascii" Hello! I have seen some SDS PAGE gels with red or orange stacking part that makes the wells visible. They use phenol red for the red stacking gel. But I couldn't find the concentration of phenol red they use for this purpose. Does anybody have any experience in this? Thanks in advance! Iraz Toprak Aydin EPFL SV ISREC, Station 19 Batiment SV, SV 2540 CH-1015 Lausanne Switzerland Tel: +41 21 693 07 36 e-mail: <mailto:[email protected]> [email protected] ------------------------------ _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods End of Methods Digest, Vol 60, Issue 11 *************************************** _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
