Align 28S sequences for different fungal species and design primers in the target region
On Thu, May 27, 2010 at 10:34 PM, <[email protected]> wrote: > Send Methods mailing list submissions to > [email protected] > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/methods > or, via email, send a message with subject or body 'help' to > [email protected] > > You can reach the person managing the list at > [email protected] > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Methods digest..." > > > Today's Topics: > > 1. Fungal 28s amplification primers (Adroit A) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 27 May 2010 07:04:08 +0530 > From: Adroit A <[email protected]> > Subject: Fungal 28s amplification primers > To: [email protected] > Message-ID: > <[email protected]> > Content-Type: text/plain; charset=ISO-8859-1 > > Dear Bionetters, > > I am trying to amplify the 28s rRNA region of a fungus. For this i already > isolated the genomic DNA and amplified it with ITS-5 and ITS-4 primers. I > got a PCR product of length approx. 700bp. However this amplified product > covers the region between 18s-5.8s-28s. Could anyone please suggest me the > universal primers that specifically amplify the 28s region alone? > > I also used ITS-1 and ITS-4 primers for amplification, however no > amplification was observed. Please let me know the reason for this. > > Thanking you in advance. > > Thanks, > Adroit > > > ------------------------------ > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > > End of Methods Digest, Vol 60, Issue 14 > *************************************** > -- Dr V K Gupta Sr Microbiologist (Mol Biology) IMBL, Department of Entomology Pun. Agric. Univ., Ludhiana (Pb)-141004- India M: 081465-55515 _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
