Hi everyone,
I realized that after making the cDNA I forgot to include a -RT
control. I used all of my RNA for the cDNA synthesis. I want to know
if there is a simple way to test if there is gDNA contamination in my
sample. I was thinking of designing primers that span an intron and
using my cDNA as template in a PCR. If there is a band on the gel,
then there is gDNA. Is this a valid way of ensuring that there is no
gDNA in my sample? And if it is, can anyone suggest a primer set I can
use for rats. (The cDNA sample is from rat brain).
-Graduate student
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