I would try APTES (3-AMINOPROPYLTRIETHOXYSILANE) coated glass. You can either make them yourself (there are a lot of protocols available online) or buy from Electon Microscopy Sciences or Ted Pella. I think the procedure takes about 3 hours. Honestly I think it's easier to buy them.
Otherwise you can try maybe GE Healthcare's PlusOne bind solution, which is another silane treatment. I think some people use them for keeping polyacrylamide gels on glass slides. Best of luck, Josh On Thu, Jul 15, 2010 at 12:23 PM, Yvonne Couch <[email protected]>wrote: > Hi all, > > I've recently started using sodium borohydride to reduce the > autofluorescence of my tissue sections. The sections are mounted on > gelatinized slides and I've noticed that they come away from the slides > when > I stain and after running some standard light-based immuno I can only > conclude this is the borohydride's fault. > > Can anyone suggest a slide coating/type of slide to stop this happening or > will I have to resort to free floating? > > Thanks > > Yvonne > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
