Daniel Prieto <[email protected]> writes: > I almost forgot... here is another extremelly interesting protocol I > haven't tried myself but my guess is that it worths the check > (http://openwetware.org/wiki/TOP10_chemically_competent_cells). Anyone > has already tried it?
I designed it, so I guess that qualifies as using it. It's pasted together from a serious many-month attempt to figure out how the companies really do things. The answer was to look at the patent literature, not the published literature. I've tried almost every chemically cocmpetent protocol out there, and this is the one which works consistently for me. Works for most other E. coli K-12 strains as well (haven't really tried on B strains). _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
