thanks a lot. but if i want to amplify the sequence, how do i design primers. do all Taq DNA polymerases have the same sequence？
CJ 2010/7/27 DK <[email protected]>: > In article <[email protected]>, jh > <[email protected]> wrote: >>Dear all, >> >>I write to search for a plasmid containing Taq DNA polymerase gene. >>Anyone who have this plasmid would be kind to help me? >>Thanks in advance. > > Have really highly competent cells and possibly try several Taq > suppliers: "transform" cells with the protein prep. Virtually every > recombinant protein prep is contaminated at a very low level with > the recombinant plasmid. Since it is not known what resistance > marker was used, plate on amp, kan and cam plates. > > I have used this trick to get several genes. > > DK > > > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
