Dear Anita, My name is Jovita Castelino and I am a PhD student at the University of Leeds. I saw your message on the bio.net forum, which was posted some years ago:
Hi, could someone give me some suggestions how to increase the yield when isolating total RNA from frozen buffy coat using the Trizol LS method? Now I add Trizol LS to the buffy coat even before I start thawing it because I am afraid RNases will work in the buffy coat. When thawed the buffy coat/Trizol LS solution is difficult to pipette, very viscous. I will be thankful for any help. Anita I wondered if you managed to solve the problem you had back then? If so, what did you do to get a better yield of RNA from frozen buffy coat? I am currently trying different kits to extract RNA from frozen buffy coat without much luck. I was thinking of trying out Trizol next but I want to make sure about it first. I would really appreicate any help/advice you could give me with this. Thank you, Jovita _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
