On 17/09/2010 15:09, Nick Theodorakis wrote:
On Sep 17, 7:59 am, WS<[email protected]> wrote:
NTC tubes? WTH is that? Neg Temp Coeff?
/Wo
I would have guessed "no template control" except that I'm not sure
how that would be different than her negative samples which she says
are clean.
Depends what you're testing for - a "negative" sample could be a
template which won't amplify.
e.g. genotyping for the presence/absence of a transgene. Positive would
be DNA from a known transgenic animal, negative would be DNA from a
known wildtype animal, no template control would have no template. In
that case the negative control would be controlling for the specificity
of the PCR in detecting the tg against genomic background, while the no
template control would be testing for contamination in one or more of
the reagents.
Magda: What size are the erroneous products? Some sort of primer dimer
is certainly a possibility.
Peter
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