Hi Kiat, when your peptide has a free amino group (N-terminal and/or lysine), it's easy:
There's a lot of reactive molecules available (eg Biotin, for immobilizing your peptide to streptavidin-beads (good for immunoprecipitation), various fluorescent labels for microscopy etc etc. The chemistry behind is simple: You may obtain the labels als NHS (N-hydroxy succinimde) esters (, sometimes also abbreviated -OSu, e.g. from Sigma, Pierce (Thermo), Molecular Probes (Invitrogen)); just dissolve them in a bit of dry DMSO or DMF and couple for 2hrs in an alkaline buffer (eg 100mM Borate, pH 8.5). To discuss details or other options (eg the amino group should not be labeled), please post your structure and what you need the label for. HTH Wo -- Neu: GMX De-Mail - Einfach wie E-Mail, sicher wie ein Brief! Jetzt De-Mail-Adresse reservieren: http://portal.gmx.net/de/go/demail _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
