Hi Eric,
of course it is possible. But I would not recommend it. You can lyse them
like this for at least 20 min (actually dependent on your volume) under
constant ice cooling. But this method is not the most effective.
I don't know if it makes sense in your case but you might want to inform
yourself about French Press or cell cracker.
Sincerely
Marcus
----- Original Message -----
From: "Eric Ma" <[email protected]>
To: <[email protected]>
Sent: Thursday, September 23, 2010 2:00 AM
Subject: Lysing E. coli cells to extract total protein
Hi guys,
I'm looking to lyse E. coli cells in order to extract total protein from
it
for downstream processing and testing. One idea that came up was to use
sonication so that we don't damage the protein - is this recommended?
Also,
if it is, do you have any recommendations on where to find protocols for
this?
I'll be looking around myself as well, but I thought I would ask others
for
advice on this too.
Thanks a lot!
Cheers,
Eric
---------------------------------------------------------------
Please consider the environment before printing this e-mail. Do you really
need to print it?
_______________________________________________
Methods mailing list
[email protected]
http://www.bio.net/biomail/listinfo/methods
_______________________________________________
Methods mailing list
[email protected]
http://www.bio.net/biomail/listinfo/methods
