Hi David, several reasons:
- no equipment (illuminator, fluorescence scanner, camera set-up) - price of dye, price of equipment - used to "old methods" (coomassie, silver) which usually are sufficient - no need to quantify proteins in gel - for real quantification, you'd always need a standard curve (dye binding properties of each protein, co-absorbance, fluorescence quenching) - existing alternatives for specific quantification like western blotting and eg antibody coated chips - are you the product manager :) suggest to cross-post in the ImageJ (http://en.wikipedia.org/wiki/ ImageJ) mailing list: [email protected] /Wo On Oct 12, 11:27 pm, David-Paul Minde <[email protected]> wrote: > > Dear all, > > has anyone of you experience with Sypro Orange for protein gel staining and > (stoichiometric) quantifications ? > I read of it in a recent BRCA2 nature article but wonder why this technique > is not much more commonly applied for all kinds of in gel protein > quantifications. Excellent combination of huge linear range, lowest > protein-protein variability, silverlike sensitivity and utmost simplicity, > fairly reasonable price seems to be pretty much unique among protein gel > stains or did I overlook any important practical drawbacks ? > Many thanks for your comments in advance ! > best, > David _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
