Hello Everyone, I was searching on the best method to purify large quantities of pure plasmid from a 250ml culture. I came across to this protocol by nature protocols ( http://www.nature.com/nprot/journal/v2/n5/full/nprot.2007.171.html) and was wondering how good it is. They report that once you make the ELP protein and its helper protein you will be able to purify up to 300ug of plasmid from 5ml culture in 30 minutes with high purity. Also, where can I find ELP-MerR fusion protein (ELP153MR) and helper protein, ELP153H6. Currently I am in Washington DC. And if anyone can advice me on what's the best method to purify large quantities of plasmid DNA, I would be appreciate it a lot. Thank you, Wadad Al Salmi _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
