Dear experts, following the standard protocol for coupling HRP to antibodies as described in Harlow & Lane's bible, the brown HRP solution turns green after adding periodate and back to brown after coupling and work-up. Anyone has a chemical explanation for that? Assuming that periodate will not reduce Fe3+ (brown) to Fe2+ (green), as it is an oxidizer, I am lacking a good explanation. Any ideas?
Thanks! Wo _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
