Hi DIEUDONNE, I've done many extractions of snail tissue, but never for a study of parasites. I don't know what tissue the schistosomes live in, but I've always dissected a little bit of foot tissue out of the snails and preserved in 95% ethanol at room temp. I remove the operculum, cut some muscle away and use that. You can also place the whole animal in 95% ethanol and dissect later -- I actually prefer to do this because the various types of tissue appear much more different from each other and are easier to identify after they have been preserved. Some snails can close the operculum so tightly that the alcohol cannot get inside the shell, though; so it's always a good idea to crack the shell with a hammer before you drop the whole thing into ethanol. Otherwise the tissue may start to deteriorate.
Best, Sandra Emme Molecular Biology Lab Researcher Florida State University ________________________________ From: Dieudonne Agudah <[email protected]> To: [email protected] Sent: Wed, January 19, 2011 5:01:28 AM Subject: schistome snail host tissue isolation, storage & DNA extraction Regards, I had been facing some difficulties with how to isolate tissue of schistosome host snail from the shell, and its preservation for optimum dna extraction anytime in future. May you kindly assist me as to how to go about this. Count greatly on your support. Sincerely, DIEUDONNE AGUDAH Parasitology Unit Noguchi Memorial Institute for Medical Research University of Ghana Legon – Accra _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
