Hi, To avoid self association or oligomer formation or aggregation you an use 2-5mmBME or DTT as a reducing agents. Further more, the best way to have single conformation of fusion protein use Gel filtration chromatography (Superdex S200) 120ml column will be better option for good separation. I don't think you are interested in removing the GST from the protein; if so use other strategies.
On 2 March 2011 01:58, Phelan, Paul J. <[email protected]> wrote: > Dear all, > This may have been asked before (maybe by me), but does anyone have any > good advice on how to disrupt GST self-association? I often have trouble > with preps. of GST-fusion proteins (when I need the intact fusion protein) > being contaminated with GST that is very difficult to get rid of. I think > this may be a result of "free" GST associating tightly with the GST-fusion. > Has anyone else had this problem, and solved it? > > Many thanks, > > Paul Phelan > Tufts University > Boston > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > -- Yours Sincerely, *Naveen Vankadari* ( 那維) Institute of Molecular Biology, Lab No. N209 ACADEMIA SINICA, _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
