Dear experts, I have question, possible somewhat related to Sudheer's, in an inversed way:
I would like to block free SH-groups in a solution that contains antibodies. I am looking for something that happily will react with free sulfhydryls, but dos not reduce disulfide bonds. I shortly thought of TCEP, but this used as reducting agent. Maleimides are used to label proteins at SH-groups - will they reduce SS bonds? What about NBD-F (Nitrobenzo-oxadiazole, a reagent for quantifying SH-groups). Iodo-acetamide? I also would like to avoid simultaneous reaction with amino groups (from eg lysines). Possible buffers are PBS, slightly alkaline borate or tris @ pH7.5. Of course, excess reagent should be easy to quench and/or remove by gel filtration. Any ideas, suggestions, comments? Thank you all, Wo . _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
