Dear Fathi, from sial.com, Product 62970: 1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus, ATCC 4698, as substrate)
Possibly you also may use some E.coli K12 strain from the molcular biology lab next door for a qualitative assay: Collect spectra time series of a defined culture with a defined amount of lysozyme to find optimal conditions. HTH Wo On 2 Mai, 10:03, Fathi Hassan <[email protected]> wrote: > Dears, > could any one kindly suggest an easy assay or protocol for lysozyme activity? > I highly appreciate your support > yours > FH _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
