Hi Sara, You might want to look at a product that might work- RapidClean from advansta.
Sia Sent from my iPhone On Jul 28, 2011, at 12:59 PM, "Sara Edge" <[email protected]> wrote: > Does anyone know of a method for removing proteoglycan contaminants from > total RNA extracts? I work with marine invertebrates (corals) and > certain species are difficult due to RNA that cannot be successfully > manipulated downstream (i.e. labeling, RT, etc.). Other research have > suggested that endogenous proteoglycans interfere with enzymes that bind > RNA. I use the TRIzol method of extraction and the Qiagen MinElute kit > for purifications. I am getting good quality RNA based on nanodrop > spectrophotometry (260:280 @ 2.0) and Agilent Bioanalyzer analysis > (clean gels, RIN @ 8-9). My 260:230's could be better and tend to be all > over the place, but have gotten up to 1.9. I would appreciate any > suggestions. > > > Thank you. > > > > Sara Edge, Ph.D. > > Marine Ecosystem Health > > Harbor Branch Oceanographic Institute at FAU > > 5600 US-1 North, Fort Pierce, FL 34946 > > 772-242-2261 > > [email protected] > > > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
