you will bite yourself in the a** when you later discover that there was a mutation somewhere else in your sequence. Considering the cost of commercial sequencing (the cheapest way are those "packages" where they just run the reaction and you get the raw data and do the evaluation by yourself, there is free software available like "finch tv"), sequencing your constructs is a must before you derive any data from them that you want to publish/patent or which are important in any other context.
I had learned my lesson once by finding out almost too late that one of the primers I had used was missing a base (still in the time of running your own DNA synthesizer and sequencing with gels and 35S) my 2c Wo On 15 Aug., 04:05, mnr mnr <[email protected]> wrote: > If I were to go down the Phusion road, will I need to sequence the entire of > my gene (4.5 kbp) or just the mutated region to make sure the protein > finally has mutated in the right places? _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
