I have seen glycerol used at 2.5% or 5% for this purpose, though I'm not sure if that was just "voodoo". I believe you can use alcohols, also? Finally, increasing alkalinity will probably affect Tm I'd say, though this would obviously impact enzyme function for PCR or restriction.
On 19 August 2011 17:13, Ma,Jin <[email protected]> wrote: > Dear Sir./Madam, > Do you know any other non or lower toxic chemicals which can attfect the Tm > of DNA? > Thanks. > Jin Ma > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > -- letters.cunningprojects.com twitter.com/onetruecathal http://www.indiebiotech.com _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
