Hi all, I'm looking for a basic protocol for nuclear and cytosolic extractions from small (c.50mg) amounts of brain tissue. I have a kit from WebScientific which doesn't appear to have enough centrifugation steps and also calls for very little tissue homogenization prior to spinning so I feel like all I am doing is breaking the tissue into small lumps and spinning it down without actually breaking it open to get the cytoplasm out.
Does anyone have a nice nuclear/cytosolic protocol with home made buffers and any ideas as to what the solution/tissue should look like at each stage? Thanks Yvonne _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
