In article <ba8be614-9f81-497a-9825-0bd24235a...@googlegroups.com>, kirankumarchi...@gmail.com says... > > I have a protein with 4 Tyr and 2 Trp residues with 17kDa. Values of > e1%, absorption coefficient of 1%(g/v)solutions of Trp and tyr in 1cm > cell at 280nm are 269.60 and83.33 respectively...so the absorption of > 1mg/ml protein solution will be how much...???? > > I got an answer of 0.5...But it is not correct...can u plz help me > solving this problem
There are two methods to calculate absorption coefficients at 280 nm if the amino acid composition of the protein is known: S. J. Perkins: Eur. J. Biochem. 157 (1986) 169-180 S. C. Gill & P. H. von Hippel: Anal. Biochem. 182 (1989) 319-326 Once you have epsilon, you only need to apply Lambert-Beer's law. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
