Irit Rappley <irapp...@scripps.edu> wrote: > Hi Sudheer, > The units for the extinction coefficient are 1/(M*cm), which match the > second value you mention. I would use that number. The calculation is > (OD 280nm) / (extinction coefficient). This will give you a concentration > in moles. You can multiply by 10^6 to get the concentration in micromoles, > which is more likely to be in the right range for your protein.
Just to give a perspective on the numbers: 1 M is 1 mole/liter. For a standard protein (Mw 40 kD) that would mean 40 kg protein/liter. This is an unrealistically high amount (slightly understated). -- Kaj _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
