Taq does not need ATP for its reaction unlike ligases. Moreover, I doubt whether it has an ATP binding motif at all. Jay
-----Original Message----- From: methods-boun...@oat.bio.indiana.edu [mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of DK Sent: Friday, February 15, 2013 7:53 PM To: meth...@magpie.bio.indiana.edu Subject: Re: Does Taq accespt ATP as substrate? In article <05d1f417-23b6-44fa-b0f7-bb0c48a1a...@googlegroups.com>, WS <novalidaddr...@nurfuerspam.de> wrote: >Dear experts, > >I am just curious if Taq would accept ATP (instead of dATP) when adding 3' > overhangs to a PCR product. I have no real idea but I'd be really surprised to find that it does. I think that it's only specialized terminal deoxynucleotidyl transferases that are promiscuous like that. DK _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
