Dear Colleagues, I have extracted genomic DNA from a local pitch Lake which appears to be of good quality and purity as the 260/280 and 260/230 ratios are good. However, the DNA is unable to be digested by the restriction enzymes xba1, bamH1 and ECOR1. This digestion step is necessary in order to proceed with the construction of a metagenomic library in supercos1 vector and xblue host. Can I get any suggestions as to overcome this problem? _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods