在 1994年2月11日星期五UTC-6下午2时07分56秒，Ross Whetten写道： > The CRC Handbook of Biochemistry gives the composition of McIlvaine buffer > as a mixture of 0.1 molar citric acid and 0.2 molar disodium phosphate. > Depending on the pH you want, you mix varying amounts of the two components > together. For example, 98 ml of citric acid + 2 ml of Na2HPO4 gives pH 2.2, > 42 ml of citric acid + 58 ml Na2HPO4 gives pH 5.6, and 2.75 ml citric acid > and 97.25 ml Na2HPO4 gives pH 8.0 (all pH determinations given at 21 C). > > > > Ross Whetten > Research Assistant Professor > Forest Biotechnology Group > North Carolina State University > Raleigh, North Carolina 27695-8008 USA > telephone or fax (919)515-7801 > e-mail rossw...@unity.ncsu.edu > -- > > > Ross Whetten > Research Assistant Professor
I am wondering what is the salt concentration of this buffer? If we perpare some standard buffer like 1 M phosphate buffer, we always adjust pH by mixing 1M acid (monobasic) with the same concentraiton of base (dibasic). Chao _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
