Plain vortexing has worked for me in the past. I have also tried squirting the sample through a small bore needle (gauge 23) using an insulin syringe, and it works, but is messier.
Hope it helps! -----Original Message----- From: methods-boun...@oat.bio.indiana.edu [mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of Phelan, Paul J. Sent: Monday, April 27, 2015 2:45 PM To: meth...@magpie.bio.indiana.edu Subject: Whole cell extract gel samples for western blot Sometimes our gel samples (whole cell extracts) for western blots are very viscous. What are the best methods for lysing small cell extracts well enough so that they don't float back out of the wells when loaded? Thank you, Paul Phelan Tufts University Boston _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
