Hi all, my lab is working on lentiviral construct that must to include in this order:
Promoter--precursor miRNA --IRES--GFP--- precursor miRNA is in the fact premiRNA plus lateral sequences but probably we cannot define as complete primary miRNA. So I don't know if at nuclear level Drosha can recognize it. My question is: in this way, if Drosha cuts, could then GFP be transported from the nucleus to cytoplasm and then translated starting from IRES? If Drosha does not cut, could this construct (premiRNA-IRES-GFP) be processed in the correct miRNA (starting from premiRNA) and it would be translated then GFP? _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
