Il giorno domenica 9 luglio 1995 09:00:00 UTC+2, s06...@aix1.uottawa.ca ha scritto: > I've been trying to think of a way to make my own TA cloning kit. > > There doesn't seem to be any available restriction enzymes that will > produce the proper ends for TA cloning (although I'm sure Invitrogen must > have one in their bag of tricks). > > I've been thinking that if I ran PCR on a linearized plasmid > (Bluescript), and used primers that annealed to each end of the plasmid > but had one or two loose A's hanging over the edge it might work. > > Any ideas out there. > > Cris Martin
Also if you could try to open a plasmid in a specific point and with a terminal transferase you could add this poliA, you should need a specific recognition site of topoisomerase... _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
