In article <ba8823a8-e4fe-4749-97c1-b2f6ab079...@googlegroups.com>, bimjhanabishwoka...@g.harvard.edu says... > > On Monday, July 2, 2007 at 7:27:59 AM UTC-4, Angela Alexander wrote: > > I'm working with a new cell line that's giving me very dilute > > lysates > > > > Speedvac'ing might be reasonable, as we have one in my lab right > > behind my bench, but I'm concerned the length of time the lysate > > will not be kept cold that will be required might not be such a > > great idea. Is dialysis the only way to go,
You can use the SpeedVac as the sample is kept cold by the evaporation of water. Just stop as soon as all water is evaporated. However, you may find chloroform/methanol precipitation (Wessel & Flügge, Anal. Biochem. 138 (1984) 141-3) or precipitation by TCA with DOC as carrier (Bensadoun & Weinstein, Anal. Biochem. 70 (1976) 241?50) much simpler. For larger samples dialysis against solid PEG is very effective (just cover the dialysis bag with PEG 20K), but make sure that you do not allow the sample to dry out completely (it's usually not possible to reconstitute if that happens). And of course, there are always centrifugal concentrators and (again, for larger volumes) Amicon ultrafiltration cells (now flogged by Millipore, IIRC). _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
