Dear Sudheer, I suppose you refer to standard sodium phosphate buffer used throughout the= biochemical literature. Both chemicals required for this recipe, NaH2PO4 a= nd Na2HPO4, are available from all major chemical supply companies that you= r university or research centre commonly orders from. The Cold Spring Harbour lab (and many biochemistry books) was so kind to su= pply a detailed recipe for the buffers depending on the desired final pH: [1]http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8303.fu ll?text_only=true good luck with your first experiments!
Dr. Hans-Georg Koen= ig Lecturer in Physiology 3D"" RCSI Physiology & Medical Physics Dept Royal College of Surgeons in Ireland 123 St. Stephen's Green, Dublin 2, Ireland T:&n= bsp;+353-1-402-2122 F:&n= bsp;+353-1- 402-2447 E:&n= bsp;hgkoe...@rcsi.ie W: [2]www.rcsi.ie RCSI DEVELOPING HEALTHCARE LEADERS WHO MAKE A DIFFERENCE WORLDWIDE -----Original Message----- From: methods-boun...@oat.bio.indiana.edu [[3]mailto:methods-boun...@oat.bio.indiana.edu] On B= ehalf Of Sudheer Sangeetham Sent: 18 September 2015 14:53 To: meth...@oat.bio.indiana.edu Subject: Na2Po2 buffer? Hi guys In one of the article which was published in 2002, I read that people used<= br> Na2Po4 buffer for their experiment. My question is Na2Po4 chemical is avail= able now ? Thank you -- Sudheer Babu.S Research Fellow Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list Methods@net.bio.net [4]http://www.bio.net/= biomail/listinfo/methods References 1. 3D"http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8303.full?te 2. 3D"http://www.rcsi.ie/"; 3. 3D"mailto:methods-bounce 4. 3D"http://www.bio.net/biomail/listinfo/methods";
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