Hi 13 M urea is in MQ. When I add 1M, 2M, 3M each, to the protein solution, the pH of the total reaction mixture is increasing with the increasing the concentration of Urea solution.
I also prepared urea in 1x PBS, behaving same. Can someone tell me solution or can some suggest strong buffer which will change the pH of the reaction mixture when i increase the concentration of urea. Thank you Regards Sudheer _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
