Hi * Strip of nickel ions by washing with 10 bed volumes of 100 mM EDTA, pH 8.
* Wash resin with 10 bed volumes of de-ionized water. * Charge resin with 2 bed volumes of 100 mM NiCl2 metal ion aqueous solution. * Wash resin with 10 bed volumes of de-ionized water to remove unbound metal ions. * Stored the resin in 30% etanol. I loaded only recharged bead on the gel to check I could see nothing on the sds-page or not, but I could see faint or visible proteins on sds page due to protein and Ni didnt strip off completely. please let me any solution for it. Thank you Regards Sudheer _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
