I am interested in assaying sphingosine from cultured cells. I have found many methods for LC-MS. Unfortunately, my lab only has a GC-MS. I have been unable to find any discussion of GC-MS methods for sphingosine.
ELISA methods exist for sphingosine-1-phosphate, and sphingosine can be phosphorylated easily enough. I could subtract endogenous S1P from total S1P, but endogenous S1P is present at much higher concentrations than sphingosine, so I'm afraid my signal would be lost in the noise. Phosphorylation with 32P followed by TLC and scintillation counting is an option, but a last resort. Are there approaches I haven't considered here, or papers where these approaches have been successfully used? _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
