Dear Morphemeticians,

I am working with landmark data for butterfly wings. Many images are from
digitized collections around the world that often use a gray background
when photographing a specimen.

However, I have noticed that many landmark studies of butterfly wings use a
light box for illuminating the wing from below to increase the contrast of
landmarks. I wonder if having a white background (versus a gray background)
makes a large difference in the ability to capture major landmarks.

Does anyone know of any references that strongly recommend the use of a
light box? Or that have collected landmarks from specimens without using a
light box? Or that speak to the accuracy of collecting landmarks from
digital images that were meant for preserving a collection of insects and
not necessarily for collecting landmark data?

Thank you for any relevant resources or suggestions.

Sincerely,
Catherine



Catherine M. Hulshof, PhD
Assistant Professor
Department of Biology
University of Puerto Rico Mayaguez
http://catherinehulshof.wordpress.com/

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