Dear, I have a question about EFDs, and was wondering if you could help me? I have performed a PCA on standardized EFDs (standardized for first harmonic, using SHAPE-software). The EFDs represented head shapes of eels which were given different diets (we compared three different diets). The results of the PCA indicated that PC1 was related to variation in head width, with high PC-scores being related to broader heads and vice versa. Next, we performed a linear regression on these PC-scores and log(length) of the three different diets and observed a size-effect in all three diets. Because of this, I wanted to divide the PC-scores by the total length of the studied eels. Then, we would perform an ANOVA on these length-corrected PC-scores between the different diets, as this would allow us to find out whether the mean head width of a certain diet is significantly broader than another diet. Therefore, my question to you is: Am I acutally allowed to divide these PC-scores by total length? Many thanks in advance! Kind regards, Jens De Meyer
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