No problem! Actually, I'm pretty new to this stuff too, so explaining kinda
helps it gel. Anyway, I'm writing something up now so I'm going to come
back to this later, but I think what you want to do to get asymmetry is run
a MANOVA or an ANOVA with size as your variable. You can do MANOVA in SPSS
and there's quite a few tutorials online for how to do that. An ANOVA is
possible in MorphoJ and probably your simplest and quickest option. Once
you have everything in go to the "Variation" tab and it will be your last
option. If you've loaded your classifiers you'll be able to assign "side"
to the box that - most helpfully - is marked "side". If that's what you're
trying to do it's quite easy. Many of the people on this list have
published on it as well so the method is very nicely supported.

Best,
Ari

On Mon, Dec 14, 2015 at 7:55 PM, Jay Devine <[email protected]>
wrote:

> Hi, Ari-
>
> Thank you so much for input. I can't believe how helpful you are, ha. Your
> advice is not basic whatsoever - I'm fairly new to this, so laying
> everything out as you did is wonderful. Essentially what I'm trying to do
> is compare bilateral asymmetry of left and right humeri in males and
> females. My classifiers are population (NP), side (R, L), specimen (random
> #), and sex (M, F). This is all part of a directed study course I'm
> currently taking, which is meant to provide me with some knowledge for my
> honours thesis. It's all a bit overwhelming, but people like you make it
> easier!
>
> Ahh, I see, I see. I don't have experience with Morphologika, but I'll try
> this out. I've used R, so perhaps I'll try using geomorph as well.
>
> Once again, I can't thank you enough.
>
> Best wishes,
>
> Jay
>
> On Mon, Dec 14, 2015 at 7:55 AM, Ariadne Schulz <[email protected]>
> wrote:
>
>> Hi Jay,
>> I'd be interested if MorphoJ had that option but I don't think it does.
>> To do it in Morphologika you'll have to first create a Morphologika file
>> which includes your wireframes. It took me a bit of doing to figure it out
>> and actually I'm wondering if one can do multiple classifiers or "labels"
>> in a Morphologika file without putting it through geomorph. But anyway,
>> I'll lay it out for you in case you don't know and also so those with more
>> experience here can critique. Basically, you just want to list things under
>> their categories as sketched out below.
>>
>> [individuals]
>> (n individuals)
>> [landmarks]
>> (n landmarks)
>> [dimensions]
>> (n Dimensions)
>> [names]
>> (Specimen_1
>> Specimen_2
>> ...)
>> [labels]
>> (name of label)
>> [labelvalues]
>> (specimen 1 label value
>> specimen 2 label value
>> ...)
>> [wireframe]
>> (1,2
>> 2,3
>> 1,3
>> ...)
>> [rawpoints]
>> '#1
>> (landmarks for Specimen 1)
>> '#2
>> (landmarks for Specimen 2)
>> ...
>>
>> I should note as it's not very clear, "labels" are your classifiers. I
>> think yours was "species" if I remember from before, so your label values
>> will be the species names. Mine is sex so my label values are "female,"
>> "possiblefemale," "unknown," "possiblemale," and "male." There's also a
>> category for "polygons" but I haven't yet gotten that to work so I won't
>> confuse matters by trying to tell you about it. Make sure all your
>> wireframe points correspond to landmarks that are included in your final
>> set in Morphologika or you will get an error. You can eliminate individuals
>> or landmarks or polygons and so forth before you get into the meat of
>> things. Once you do a Procrustes fit and PCA, you'll want the TPS tab in
>> the control panel. Sometimes the image comes up blank, but if you just
>> click on it it will solve itself. Morphologika also allows you to "Explore
>> Space" which is really helpful. Make sure that button in the Control Panel
>> is toggled and then click on any spot in the PC chart. You'll note that the
>> landmarks in the viewer change to correspond with the position in the PC
>> chart you've chosen.Given what you're trying to do though, I think you
>> might want to try geomorph in R. It's really quite versatile if a little
>> daunting. I hope this wasn't too basic ... let me know how you get on.
>>
>> Best,
>> Ari
>>
>> On Mon, Dec 14, 2015 at 5:18 AM, Jay Devine <[email protected]>
>> wrote:
>>
>>> Hello again, Morphmet!
>>>
>>> I received an immense amount of help with my last question, so I figured
>>> I would try again! My silly question this time concerns 3D visualization of
>>> shape change.
>>>
>>> I've been able to successfully create wireframe graphs for my data, but
>>> for some reason I can not create transformation grids. I'm aware that you
>>> need to right-click on the "PC shape changes" background to change the type
>>> of graph. Unfortunately, transformation grids doesn't appear as an option
>>> for me to choose. Is there perhaps a preliminary step I need to do?
>>>
>>> Does anyone know why this could be happening?
>>>
>>> Thanks in advance for your time and consideration.
>>>
>>> Respectfully,
>>>
>>> Jay
>>>
>>> --
>>> MORPHMET may be accessed via its webpage at http://www.morphometrics.org
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>>
>>
>

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