Chuming,

mpiblast-pio 1.4.0 uses the same mpiformatdb as mpiblast-1.4.0 does, so
you can reuse the database fragments created by mpiblast 1.4.0. One
thing you might want to know is that if you are using NFS, make sure to
add "--use-master-write" option to run mpiblast. You can also look at
the README file in the mpiblast-pio package for more information about
the mpiblast-pio specific options.

Thanks,
Heshan

On Tue, 2006-03-21 at 13:43 -0500, Chuming Chen wrote:
> Heshan,
> 
> Thank you very much.
> 
> I have successfully compiled mpiblast-pio 1.4.0 with Oct 2004 NCBI
> toolbox.
> 
> Do I still need to run mpiformatdb of mpiblast-pio first, then run
> mpiblast?
> 
> Chuming
> 
> On 3/20/06, [EMAIL PROTECTED] <[EMAIL PROTECTED]> wrote:
>         Chuming,
>         
>         The current release of mpiBLAST-pio only supports the Oct2004
>         NCBI
>         toolbox.  Could you give it a try and let me know what is
>         happening? Sorry
>         about the inconvenience. We are working on it and will
>         integrate the 
>         newest version of NCBI toolbox in the next release.
>         
>         Thanks,
>         Heshan
>         
>         > I followed the compilation order you mentioned, still no
>         luck. By the way,
>         > I
>         > am compiling mpiBLAST-pio 1.4.0 instead of mpiBLAST 1.4.0.
>         >
>         > myblastool.c: In function `BLASTOptionNewEx':
>         > myblastool.c:1462: error: structure has no member named
>         `no_check_score'
>         > myblastool.c:1486: error: structure has no member named
>         `no_check_score' 
>         >
>         > The errors above happend for the structure
>         "BLAST_OptionsBlkPtr options".
>         >
>         > Do you know where  is this structure defined?
>         >
>         > Thanks,
>         >
>         > -Chuming
>         >
>         > On 3/20/06, Anoop Rajendra <[EMAIL PROTECTED]> wrote:
>         >>
>         >> Did you compile the NCBI source tree first? You'll need to
>         do that
>         >> before 
>         >> you compile mpiblast.
>         >> The order of compiling is
>         >>
>         >> 1. Patch NCBI Blast with the patch supplied by mpiblast
>         >> 2. Compile NCBI Blast
>         >> 3. Compile MPI-Blast and during configure point to the NCBI
>         source tree 
>         >> that you just compiled.
>         >>
>         >> -Anoop
>         >>
>         >> On Mar 20, 2006, at 2:06 PM, Chuming Chen wrote:
>         >>
>         >> I did apply the ncbi_Dec2005_evalue.patch. But I still got
>         the same 
>         >> error
>         >> messages.
>         >>
>         >> On 3/20/06, Anoop Rajendra < [EMAIL PROTECTED]>
>         wrote:
>         >> >
>         >> > If you're compiling with the latest NCBI source tree, you
>         will want 
>         >> > to use ncbi_Dec2005_evalue.patch
>         >> > rather than the june 2005 patch.
>         >> >
>         >> > -Anoop
>         >> >
>         >> > On Mar 20, 2006, at 1:18 PM, Chuming Chen wrote: 
>         >> >
>         >> > > Hi all,
>         >> > >
>         >> > > I failed to compile mpiblast-pio-1.4.0 on Rocks cluster
>         4.1.
>         >> > >
>         >> > > Here is the error messages I got: 
>         >> > >
>         >> > > myblastool.c: In function `BLASTOptionNewEx':
>         >> > > myblastool.c:1462: error: structure has no member named
>         >> > > `no_check_score'
>         >> > > myblastool.c :1486: error: structure has no member
>         named
>         >> > > `no_check_score'
>         >> > > myblastool.c: In function `BLASTPostSearchLogic':
>         >> > > myblastool.c:6263: error: structure has no member
>         named 
>         >> > > `original_expect_value'
>         >> > > myblastool.c:6269: warning: assignment from
>         incompatible pointer
>         >> type
>         >> > > make[2]: *** [myblastool.o] Error 1
>         >> > > make[2]: Leaving directory `/home/chen/src/mpiblast-
>         1.4.0-pio/src'
>         >> > > make[1]: *** [all] Error 2
>         >> > > make[1]: Leaving directory
>         `/home/chen/src/mpiblast-1.4.0-pio/src'
>         >> > > make: *** [all-recursive] Error 1
>         >> > > 
>         >> > > Am I missing anything? I have NCBI src tree and have
>         applied the
>         >> > > ncbi_Jun2005_evalue_patch.
>         >> > >
>         >> > > Thank you for your help.
>         >> > > 
>         >> > > Chuming Chen
>         >> >
>         >> >
>         >> >
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