Alertmanager version?

Try logging into the same host where alertmanager runs, and running the 
following:

openssl s_client -connect smtp-relay.gmail.com:587 -starttls smtp
ehlo mydomain.com
mail from:<[email protected]>

See if you get an error like this:

550-5.7.0 Mail relay denied [<address>]. Invalid credentials for
550-5.7.0 relay for one of the domains in: mydomain.com (as obtained from 
HELO and
550-5.7.0 MAIL FROM).
550-5.7.0 Email is being sent from a domain or IP address which isn't 
registered
550-5.7.0 in your G Suite account. Please login to your G Suite account and
550-5.7.0 verify that your sending device IP address has been registered 
within
550-5.7.0 the G Suite SMTP Relay Settings. For more information, please 
visit
550 5.7.0  https://support.google.com/a/answer/6140680#maildenied

One possibility is that your host has gained a new external IP address (on 
IPv6 perhaps?) which has not been registered.

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