Hello, Marko,

Think you for your reply. I can use the bar object "Action" > "Levels" to set 
the levels/scales to -100~100, but could you tell me what are the units for the 
values?

Best regards
------------------------------------------------------------------
From:Marko Hyvonen <mh...@cam.ac.uk>
Sent At:2019 Sep. 12 (Thu.) 15:58
To:pymol-users <pymol-users@lists.sourceforge.net>
Subject:Re: [PyMOL] How to manually define the lower and upper limit of vacuum 
electrostatics?

 Hi Yeping, 

 Click on the bar object "Action" > "Levels" for a few preset levels/scales.

 hth, Marko 

On 12/09/2019 08:03, sunyeping via PyMOL-users wrote:
Dear all,

I am trying to compare the vacuum electrostatics of three related proteins. I 
loaded them in pymol and use "Action>generate>vacuum electrostatics menu to 
generate the vacuum electrostatics of the three proteins. I find that the lower 
and upper limit of the gradient bars generated with the  vacuum electrostatics 
of the three proteins are different. They are -64.1~64.1, -72.2~72.2 and 
-82.2~82.2, respectively.  

I wonder whether I could define the lower and upper limit of the gradients to 
the same values so that the vacuum electrostatics of the three proteins can be 
better compared?

Best regards.
Yeping 

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Marko Hyvonen
Department of Biochemistry, University of Cambridge
mh...@cam.ac.uk
+44 (0)1223 766 044
@HyvonenGroup
http://hyvonen.bioc.cam.ac.uk
 



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