Hi,
Thank you for a reply.
I am not working with cryo-EM but it's an X-ray diffraction map.
A similar kind of problem I am facing in chimera, so what could be done?

Thank you

Shubhashish Chakraborty
PhD Research Scholar (SRF)
Varma Lab
Structural and Molecular biology Lab
Advanced Centre for Treatment, Research and Education in Cancer (ACTREC)
Khargar, Navi Mumbai




On Mon, Apr 12, 2021 at 12:42 PM Tamas Hegedus <biohege...@gmail.com> wrote:

> Hi,
>
> PyMOL seems not to primarily target working with densities.
>
> I suggest to use Chimera if you work with cryo EM maps.
> E.g. you can use it to fit your struct into the density map.
> If you decide to use Chimera, be careful, a first step of frustration
> could be caused by the extension of the density file. Yes, the extension -
> .mrc or .map; depending on the extension the map is aligned to the origo or
> not (or something like that).
>
> Bests,
> Tamas
>
> On 4/12/21 8:34 AM, shubhashish chakraborty wrote:
>
> Hello,
> I am not able to align my pdb file with its respective electron density
> map generated by CCP4i. Kindly let me know what is going wrong.
> An image is attached for better reference.
>
> Thank you
>
> Shubhashish Chakraborty
> PhD Research Scholar (SRF)
> Varma Lab
> Structural and Molecular biology Lab
> Advanced Centre for Treatment, Research and Education in Cancer (ACTREC)
> Khargar, Navi Mumbai
>
>
>
>
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