Wrong list! This does not concern R programming.

Post on the r-sig-mixed-models list instead in **PLAIN TEXT** rather than html.

Cheers,
Bert

Bert Gunter
Genentech Nonclinical Biostatistics
(650) 467-7374

"Data is not information. Information is not knowledge. And knowledge
is certainly not wisdom."
H. Gilbert Welch




On Thu, May 22, 2014 at 6:52 AM, Claire <c.word...@live.com> wrote:
> Dear all,
>
> I am quite new to R so apologies if I fail to ask properly. I have done a 
> test comparing bat species richness in five habitats as assessed by three 
> methods. I used a linear mixed model in lme4 and got habitat, method and the 
> interaction between the two as significant, with the random effects 
> explaining little variation.
>
> I then ran Tukey's post hoc tests as pairwise comparisons in three ways:
>
> Firstly in lsmeans:
> lsmeans(LMM.richness, pairwise~Habitat*Method, adjust="tukey")
>
> Then in ‘agricolae’:
>
> tx <- with(diversity, interaction(Method, Habitat))
> amod <- aov(Richness ~ tx, data=diversity)
> library(agricolae)
> interaction <-HSD.test(amod, "tx", group=TRUE)
> interaction
>
> Then in ghlt 'multcomp':
> summary(glht(LMM.richness, linfct=mcp(Habitat="Tukey")))
>
> summary(glht(LMM.richness, linfct=mcp(Method="Tukey")))
>
> tuk <- glht(amod, linfct = mcp(tx = "Tukey"))
> summary(tuk)          # standard display
> tuk.cld <- cld(tuk)   # letter-based display
> opar <- par(mai=c(1,1,1.5,1))
> par(mfrow=c(1,1))
> plot(tuk.cld)
> par(opar)
>
> I got somewhat different levels of significance from each method, with ghlt 
> giving me the greatest number of significant results and lsmeans the least. 
> All the results from all packages make sense based on the graphs of the data.
>
> Can anyone tell me if there are underlying reasons why these tests might be 
> more or less conservative, whether in any case I have failed to specify 
> anything correctly or whether any of these post-hoc tests are not suitable 
> for linear mixed models?
>
> Thankyou for your time,
> Claire
>
>         [[alternative HTML version deleted]]
>
>
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>

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