Thanks Bert,

Will post on r-sig-mixed-models list. Can't help it being in html though as i 
sent the query via -email.

Cheers
Claire

> Date: Thu, 22 May 2014 09:29:44 -0700
> Subject: Re: [R] Post-hoc tests on linear mixed model give mixed results.
> From: gunter.ber...@gene.com
> To: c.word...@live.com
> CC: r-help@r-project.org
> 
> Wrong list! This does not concern R programming.
> 
> Post on the r-sig-mixed-models list instead in **PLAIN TEXT** rather than 
> html.
> 
> Cheers,
> Bert
> 
> Bert Gunter
> Genentech Nonclinical Biostatistics
> (650) 467-7374
> 
> "Data is not information. Information is not knowledge. And knowledge
> is certainly not wisdom."
> H. Gilbert Welch
> 
> 
> 
> 
> On Thu, May 22, 2014 at 6:52 AM, Claire <c.word...@live.com> wrote:
> > Dear all,
> >
> > I am quite new to R so apologies if I fail to ask properly. I have done a 
> > test comparing bat species richness in five habitats as assessed by three 
> > methods. I used a linear mixed model in lme4 and got habitat, method and 
> > the interaction between the two as significant, with the random effects 
> > explaining little variation.
> >
> > I then ran Tukey's post hoc tests as pairwise comparisons in three ways:
> >
> > Firstly in lsmeans:
> > lsmeans(LMM.richness, pairwise~Habitat*Method, adjust="tukey")
> >
> > Then in ‘agricolae’:
> >
> > tx <- with(diversity, interaction(Method, Habitat))
> > amod <- aov(Richness ~ tx, data=diversity)
> > library(agricolae)
> > interaction <-HSD.test(amod, "tx", group=TRUE)
> > interaction
> >
> > Then in ghlt 'multcomp':
> > summary(glht(LMM.richness, linfct=mcp(Habitat="Tukey")))
> >
> > summary(glht(LMM.richness, linfct=mcp(Method="Tukey")))
> >
> > tuk <- glht(amod, linfct = mcp(tx = "Tukey"))
> > summary(tuk)          # standard display
> > tuk.cld <- cld(tuk)   # letter-based display
> > opar <- par(mai=c(1,1,1.5,1))
> > par(mfrow=c(1,1))
> > plot(tuk.cld)
> > par(opar)
> >
> > I got somewhat different levels of significance from each method, with ghlt 
> > giving me the greatest number of significant results and lsmeans the least. 
> > All the results from all packages make sense based on the graphs of the 
> > data.
> >
> > Can anyone tell me if there are underlying reasons why these tests might be 
> > more or less conservative, whether in any case I have failed to specify 
> > anything correctly or whether any of these post-hoc tests are not suitable 
> > for linear mixed models?
> >
> > Thankyou for your time,
> > Claire
> >
> >         [[alternative HTML version deleted]]
> >
> >
> > ______________________________________________
> > R-help@r-project.org mailing list
> > https://stat.ethz.ch/mailman/listinfo/r-help
> > PLEASE do read the posting guide http://www.R-project.org/posting-guide.html
> > and provide commented, minimal, self-contained, reproducible code.
> >
                                          
        [[alternative HTML version deleted]]

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