Thank you Peter and John!

On Fri, Sep 11, 2015 at 5:30 AM, John Marshall <[email protected]> wrote:
> On 10 Sep 2015, at 21:54, Terry Casstevens <[email protected]> wrote:
>> If you use bgzip to zip an already gzipped file, does that result in a
>> correctly bgzipped file?
>>
>> Or should you first ungzip the file and then bgzip?
>
> Your tools are expecting a file with a single layer of bgzipping, so as Peter 
> noted you will need to undo the existing plain compression and then re-bgzip 
> it.  This can be done without an uncompressed temporary file with
>
>         mv foo.vcf.gz foo.vcf.plaingz
>         gzcat foo.vcf.plaingz | bgzip > foo.vcf.gz
>
> and the final foo.vcf.gz will be identical to what you'd get with "gunzip 
> foo.vcf.gz; bgzip foo.vcf" [1].
>
>     John
>
> [1] Format nerdery: because bgzip doesn't ever put any filenames in the GZIP 
> header.
>
> --
>  The Wellcome Trust Sanger Institute is operated by Genome Research
>  Limited, a charity registered in England with number 1021457 and a
>  company registered in England with number 2742969, whose registered
>  office is 215 Euston Road, London, NW1 2BE.

------------------------------------------------------------------------------
_______________________________________________
Samtools-help mailing list
[email protected]
https://lists.sourceforge.net/lists/listinfo/samtools-help

Reply via email to