[Histonet] Histologist needed UAMS Little Rock AR
Please no recruiters reply to this. We are a state University and we are not allowed to use your services. I am sorry however; this saves us both time. We currently have an opening for a registered HT or HTL at the University of Arkansas for Medical Sciences in Little Rock AR. It is a full time position with full benefits, vacation, sick time and 12 paid holidays per year. We are fast growing Histology Laboratory with new equipment and procedures coming for more experience with growth potential. The position is listed under UAMS Jobs as # 50006966. If you have any question you may call me at the number below. I will be happy to discuss the position and future plans with you. Best Regards, Pamela A Marcum AP Supervisor Histology Slot 502 4301 W Markham Street Little Rock AR 72205 Office: 501-686-7554 Fax: 501-686-7151 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Collagen type IV
Hello: Is anybody using collagen IV antibody as a marker for basal lamina in breast tissue? Thanks, Marcela Bertola B.S., HTL(ASCP), QIHC Bridgeport Hospital Bridgeport, CT,USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histologist job opening in Red Bank, NJ
I have an opening for a Histologist to work full time, M-F for a doctor's office in Red Bank, NJ. Please call me or email me at sha...@rotator.netmailto:sha...@rotator.net if you are interested in applying for the position. Happy New Year, Sharon SHARON GREEN WEINREB Rotator/Medical/Industrial Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 732-353-4292 direct phone 732-238-6050 office phone 732-238-2152 fax sha...@rotator.netblocked::mailto:sha...@rotator.net www.rotator.nethttp://www.rotator.net/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Shatter effect on specimens
Does anyone in histoland have corrected problems with shattered biopies, EM ect? Thanks for your help. Jimmy Jimmy Lofton, M.S., HT,CT(ASCP) Manager Histology Laboratory Holy Cross Hospital 1500 Forest Glen Road Silver Spring, MD 20910-1484 301-754-7353 (Phone) 301-754-8563 (Fax) lofto...@holycrosshealth.org Trinity Health MailGate made the following annotations - CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and privileged under state and Federal privacy laws. If you received this e-mail in error, be aware that any unauthorized use; disclosure, copying, or distribution is strictly prohibited. Please contact the sender immediately and destroy all copies of this message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Saturday Coverage Orange County California
We are in need of a per diem histotech who can work on Saturday mornings. We are located in Fountain Valley, California. Please send me an email if interested or fax resume to: 714 755-2984 Thank you, Paula Lucas ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Shatter effect on specimens
Chatter or shatter? Chatter to me is a microtomy problem more so, shatter I would look more to processing and handling... but people use those descriptions to mean both sometimes, but here are my thoughts #1 tissue type considerations - GI Bx and liver Bx especially- I have seen this alot when they get processed all together with other tissues( in a do everything type of program). #2- over dehydration, too prolonged or not enough gradation from dilute to absolute ETOH for certian tissue types( biopsies here) in the tissue processing program espeically those types in #1. #3 sometimes also more problems when rapid processing used with MW or when they had rapid processors with acetone espeically( gone now I think, the original Sakura), but the program just needs to be optimized if that is the case. #4 poor microtomy technique, and/or loose blade holder, block holder (due to rusted, worn out screws, or springs ) especially the little ones in the blade holder, or just not being clamped properly, or the angle can be off- this is the chatter. Sometimes something is loose or worn, but some people clamp everything super-tight, and this is sometimes trouble too, or can't correct their angles. #5- I guess you would need to figure out what the source is intially to correct. But all could possibly be corrected with program changes to processing, microtome PM, training of microtomist to recognize the artifact on waterbath before it gets to the slide, and get more comfortable/confident with their microtome. At the bench I can correct the less than ideal processing sometimes when already crunchy by somewhat prolonged soaking in icy water before sectioning, and trying to section a little slower than I normally would. Can't help much with EM, not my experience, but I would guess some of the same issues, and/or vibration on the ultramicrotome?Joelle Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver Date: Thu, 29 Dec 2011 09:35:10 -0500 From: lofto...@holycrosshealth.org To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] Shatter effect on specimens Does anyone in histoland have corrected problems with shattered biopies, EM ect? Thanks for your help. Jimmy Jimmy Lofton, M.S., HT,CT(ASCP) Manager Histology Laboratory Holy Cross Hospital 1500 Forest Glen Road Silver Spring, MD 20910-1484 301-754-7353 (Phone) 301-754-8563 (Fax) lofto...@holycrosshealth.org Trinity Health MailGate made the following annotations - CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and privileged under state and Federal privacy laws. If you received this e-mail in error, be aware that any unauthorized use; disclosure, copying, or distribution is strictly prohibited. Please contact the sender immediately and destroy all copies of this message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C4d immunofluorescent positive controls
We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies. Thanks in advance for any and all responses. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: C4d immunofluorescent positive controls
Do you do renal transplant? If yes, look for renal biopsies submitted for immunofluorescence studies, Let the Pathologists confirm that the biopsy is acute cellular rejection from renal transplant, because acute cellular rejection from kidney transplant is known to be positive for c4d. After the studies, cut some extra frozen sections from this biopsy and fix in acetone for 20 mins, and cover the slide with parafilm to avoid cracking of slide and store in the freezer. They are good for some months. Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel:432-640-2348 Fax:432-640-2303 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, December 29, 2011 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d immunofluorescent positive controls We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies. Thanks in advance for any and all responses. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotechnician or Histotechnologist Needed for Opening in Naples, FL
Allied Search Partners is searching for a qualified Histotech to work in Naples, FL. To apply please send resume to bran...@alliedsearchpartners.com mailto:bran...@alliedsearchpartners.com Location: Naples, FL Job Title: Histotech Job Description: · Receives and accesses all tissue, surgical specimens, foreign bodies, orthopedic devices, and other specimens removed at surgery and submitted from the operating room. · Biopsies, aspiration specimens, and cytology specimens are also received and accessioned from the emergency room, nursing units, or outpatient procedures. · Arranges for the submission of various tissues · Maintains and relates supply requirements to the Assistant Laboratory Manager. · Stores and disposes of specimens, paraffin blocks, and slides appropriately · Assists the Pathologist in processing tissue for immediate frozen sections. · Reports to an appropriate Section Supervisor or the Assistant Laboratory Manager, and is ultimately responsible to the Laboratory Manager/Pathologist. Requirements: Florida Clinical Lab or Histology License -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with remove. Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com http://www.alliedsearchpartners.com/ Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 http://www.alliedsearchpartners.com/ This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CPT CODE 88106 and 88104
PROCEDURE CODES FOR NON-GYNECOLOGIC, NON-FINE-NEEDLE ASPIRATION SPECIMENS 88104: Direct smear 88108: Concentrated prep (cytospin or Saccomanno) 88112: Enriched or concentrated prep (e.g., ThinPrep(r), SurePath(r)) This information was taken from Cibas Ducatman's Cytology: Diagnostic Principles and Clinical Correlates As for code 88106, I'm not sure what simple filter method they are referring to (Millipore?) but if you check out CAP's website, it seems they don't either. :) http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtlt%7BactionForm.contentReference%7D=cap_today%2Ffeature_stories%2F0107CPT.html_state=maximized_pageLabel=cntvwr Amber Amber M. Fimbres, MHA, CT(ASCP)CMHTLCM Cytotechnologist UC Irvine Medical Center -Original Message- From: Tunde Ajibade [mailto:tajib...@echd.org] Sent: Wednesday, December 28, 2011 10:47 AM To: histonet@lists.utsouthwestern.edu; Tunde Ajibade Subject: [Histonet] CPT CODE 88106 and 88104 Hello everyone, I need more information about the following CPT: CPT 88106-Cytopathology, fluids, washing or brushings, except cervical or vaginal: SIMPLE FILTER METHOD with interpretation CPT 88104 -Cytopathology, fluids, washing or brushings, except cervical or vaginal: smears with interpretation. My question is that, what is the SIMPLE FILTER METHOD means for CPT 88106? Does it means making of thin prep from the specimen? Which one is more appropriate to use? I need help. Thanks Tunde Ajibade BS, HTL(ASCP)QIHC CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 97, Issue 32
Gui, I don't see any problem with the protocol. Here's what I would do before waisting my time on the ICC staining. I check the viability of the cells before staining, because many thing can go wrong with the primary culture (my guest it's not cell line, am I right?). - To do this, at the end of culturing, I rinse off the medium with pre-chilled PBSwithout any ionic detergent (ie. Triton, Tween 20, etc). - Add DAPI/PBS (your working concentration is fine) for 5 min @ RT. - Wash off excess DAPI with PBS x3. - Take the cells check under the fluorescent microscope if the NUCLEI are in good condition (with proper filter). They should be the same morphology as your 1st day culture after they adherent. ** If they look the same as your ICC, then that mean it's not the protocol problem. It's your tissue culture problem. Primary rodent brain culture are Not easy to culture. Please let me know if my suggestion are helping or not. Good Luck! Madeleine Huey BS, HTL/QIHC (ASCP) Supervisor-Pathology (IPOX Histology) El Camino Hospitial madelein...@elcaminohospital.org On Thu, Dec 29, 2011 at 10:00 AM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. pap supply processing (Gale Limron) 2. CPT CODE 88106 and 88104 (Tunde Ajibade) 3. RE: Histonet Digest, Vol 97, Issue 31 (Goodwin, Diana) 4. Shatter effect on specimens (Jimmy Lofton) 5. Histologist needed UAMS Little Rock AR (Marcum, Pamela A) 6. Saturday Coverage Orange County California (Paula Lucas) 7. RE: Shatter effect on specimens (joelle weaver) 8. C4d immunofluorescent positive controls (Martha Ward-Pathology) 9. RE: C4d immunofluorescent positive controls (Tunde Ajibade) -- Message: 1 Date: Wed, 28 Dec 2011 13:06:13 -0500 From: Gale Limron ga...@unionhospital.org Subject: [Histonet] pap supply processing To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: d7b361aaaf4a8895e5918634850a04d98f1...@uhexg-3.unionhospital.org Content-Type: text/plain; charset=us-ascii Happy Holidays! Can anyone tell me what their current per-test pricing is for ThinPrep pap supplies? I'm putting together a study to present numbers to possibly bring our Gyn Cytology back in-house. Thank you, Gale Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. -- Message: 2 Date: Wed, 28 Dec 2011 12:46:50 -0600 From: Tunde Ajibade tajib...@echd.org Subject: [Histonet] CPT CODE 88106 and 88104 To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, Tunde Ajibade tajib...@echd.org Message-ID: abf9e8674bbe5448920911351ac6d3e50208fb5...@mchmail.echd.org Content-Type: text/plain; charset=us-ascii Hello everyone, I need more information about the following CPT: CPT 88106-Cytopathology, fluids, washing or brushings, except cervical or vaginal: SIMPLE FILTER METHOD with interpretation CPT 88104 -Cytopathology, fluids, washing or brushings, except cervical or vaginal: smears with interpretation. My question is that, what is the SIMPLE FILTER METHOD means for CPT 88106? Does it means making of thin prep from the specimen? Which one is more appropriate to use? I need help. Thanks Tunde Ajibade BS, HTL(ASCP)QIHC CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you
