[Histonet] Help for identifying the blue stained structure

2014-05-17 Thread Rui TAHARA


Hello, 
I have an embryonic sample that
decalcified, paraffin embedded, and stained with Mallory Trichrome (Aniline
Blue, Orange G, Acid Fuchsin). I will upload the image in the Histonet Images. 
This
is a cranial region where the bone is being resorbed, so I expected to see the
bone (dark blue in trabecular), and red blood, and adjacent white spaces that
is being resorbed. Instead, there is a very uniform, granular structure stained
with blue without any nuclei at the resorbed regions. This structure looks like
almost crystal or some kind of secretion leakage from the ossifying bone. I
need a help to identify this blue stained things. I don’t think this is osteoid,
because at later stage embryos, there is no bone at this region. 

If you have some suggestions for other
stain to identify this blue thing, or help identifying this, I really
appreciate it. 

 

Thank you in advance, 

 

Rui 

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[Histonet] time frame on a CLIA application in colorado

2014-05-17 Thread Gloria Cole
Hello,

 

I have been striking out on getting the local CLIA office to call me back I
regards to some information.  I am turning to you all in hopes someone in
the Denver Colorado area can help me. I need to find out the time frame it
takes from the time a CLIA application (high complexity lab and assuming all
information is filled out correctly) is submitted to the time a coupon for
payment  is received in the mail? 3 months? 6 months?   If anyone can help
me find this information, I would appreciate it. 

 

Thanks, 

Gloria

 

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[Histonet] Re: Histonet Digest, Vol 126, Issue 15

2014-05-17 Thread Amos Brooks
Hi Andi,
 I have a Google Doc of our procedure. I just sent you an invite to
view it. If anyone else wants to see it, just let me know I'll share :-) I
know the procedure calls for overnight in the LFB solution. It's for the
best, the results are much better if you are patient. It is really
important to not run the rehydration to water. End in 95% ETOH, the water
will really kill the stain.

Good luck,
Amos


On Thu, May 15, 2014 at 1:00 PM,
wrote:

> Message: 7
> Date: Wed, 14 May 2014 19:23:58 +
> From: "Grantham, Andrea L - (algranth)" 
> Subject: Re: [Histonet] Luxol Fast Blue
> To: Christina Kreutzer 
> Cc: ""
> 
> Message-ID: 
> Content-Type: text/plain; charset="us-ascii"
>
> There are several procedures online. Just google Luxol fast blue staining
> procedures. It is an easy stain to do.
>
> Andi
>
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