Re: [Histonet] guinea pig IHC
Especially a very blood tissue like GP spleen. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: Patsy Ruegg Sent: Thursday, January 31, 2019 11:51 AM To: Jan Shivers; histonet Subject: Re: [Histonet] guinea pig IHC In my experience it is not that GP have a higher peroxidase level, it is frozen sections in general that cannot be blocked with h202, unless they are fixed for a long time in formalin. What are others experiences with h202 blocking on frozen sections. I always used an IHC detection system that did not require h202 blocking for frozen sections. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: Jan Shivers Sent: Tuesday, January 29, 2019 12:58 PM To: histonet Subject: [Histonet] guinea pig IHC Has anyone ever performed IHC on frozen sections of guinea pig tissue? I am experiencing an enormous amount of bubbling when doing the peroxidase blocking step, even though I'm only using a 0.3% concentration of H2O2. And when I say 'enormous', I mean it's like continuous champagne bubbles rising out of the tissue, even after 20 minutes in the H2O2 solution. I can't find anything in the literature that mentions guinea pigs having a higher peroxidase content in their tissues. Thanks for any help that anyone can provide. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cytology listserv
Haven't seen any cytology listservs except the one for members of the ASC. There are some cytology facebook pages where you could get questions answered. This Histonet listserv is very informative. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cytology listserv
Hello- I know many of us have overlap between histology/cytology/cytology prep. Wondering if there is a helpful listserv available like this - or is my best chance to bring my questions here? Thanks Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: [External Sender] Re: bleach under the sink
-Original Message- From: Hannen, Valerie Sent: Friday, February 01, 2019 5:47 AM To: 'Terri Braud' Subject: RE: [External Sender] Re: [Histonet] bleach under the sink We are not allowed to store anything under our sinks, the Compliance department has told us that it is an OSHA requirement. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.com www.parrishmed.com -Original Message- From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, January 31, 2019 2:04 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [External Sender] Re: [Histonet] bleach under the sink WARNING: This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Cleaning supplies may be stored under the sink, but since "things" have a way of migrating there as well, we just have a blanket rule, nothing under the sink. Of course, this would never occur in the Histology Department, LOL. The space is actually locked off. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Histotechnologist Needed near Philly! (Kristyn Ferber) 2. Bleach Regs (Maryann Deathridge) 3. Re: Bleach Regs (Laurie Redmond) 4. Re: Bleach Regs (Morken, Timothy) Message: 2 Date: Thu, 31 Jan 2019 15:11:45 + From: Maryann Deathridge Subject: [Histonet] Bleach Regs Hello Histonetters! Has anyone heard of a regulation by CAP, CLIA or OSHA regarding commercial bleach stored under the laboratory sink. My lab routinely has a gallon container of dated/ bio-labeled commercial bleach stored under the sink for cleaning purposes. Daily use to clean special stain coplin jars,etc.. I had a suspicious med tech tell my pathologist that is was against regulations to have it in the lab anytime ! HUH Responses welcomed. Have an awesome Thursday! Maryann Deathridge, Lab Manager Pathology Assoc. of St. Thomas 4220 Harding Pike Bldg. SE, Suite 504 Nashville, TN 37205 madeathri...@pastnashville.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet == "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
