[Histonet] IHC CRO
Hello, Does anyone has a list of category can share that is used for IHC CRO comparison? I want to make a table to do comparison after talking to multiple CROs and I don't want to miss something. Just wondering if anyone did this before and can share your table or list? Thanks, Wendy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Galectin 9 IHC
Hello histonetters, Has anyone done Galectin 9 IHC on human FFPE tissue? Which antibody you recommend? Or do you know any CRO that has this Gal 9 IHC service? Thanks, Wendy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NIS Elements
Hello, We used to use Image-Pro Premier to capture and analyze images. Does anyone use NIS Elements with Nikon to do image capture and analyze? How do you feel about it? If you used both of them, could you let me know what you think? We need to make a decision for a new purchase. Thank you, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] process formalin-fixed tissues from animals infected with a virus
Hello, Does anyone know what we should know about process formalin fixed animal tissue that has been infected with virus? What certificate/requirement/qualification we should have? We have never done this before and need to make decision if we should do it or not. Thank you very much, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Victoria Blue for lung tissue
Hello Histonet, I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Collagen Hybridizing Peptide
Hello histonet, Does anyone ever do this on FFPE tissue? How you feel about this technique and what I should pay attention to? Any commend appreciated. Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] test
Hello, I am just wondering if I myself will see the email I sent out to histonet. Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] rodent heart tissue harvesting
Hello histonet, My coworker wants me to ask experts here the best way to use Potassium Chloride during rodent heart harvesting. Could any one share your procedure? We searched around on internet but not much help. If there's any way other than Potassium Chloride to relax heart muscle, please advice too. Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks!
Thanks to all who responded to my question regarding PAS staining! We got much better staining after following you guys suggestion: a. we purchased reagent from American Mastertech b. we used 1% periodic acid c. we rinse slides under warm water after Schiff's step. Thanks again, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAS staining
Hello, We tried to do PAS staining on kidney. I attached my picture here. I am looking for the staining results that has more reddish as showing in the second picture. So I can do quantification better. We purchased Schiff's reagent from Sigma freshly. Could anybody teach us how can I can the staining like that? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] type IV collagen antibody
Hello histonet, Could anyone recommend a type IV collagen antibody work on FFPE mouse tissue? Thanks in advance, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] test
___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] frozen tissue training
Hello histonet, I am looking for a frozen tissue training session for my new employee. It includes frozen tissue harvest, storing, sectioning, etc. Could any one recommend a training session inside US? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GPT2/ALT2 antibody
Hello histonet, It seems my last sent out email got lost. So I have to send it again. Could anyone recommend an GPT2/ALT2 antibody works on rat paraffin section please? I did some search but don't know which one is good. Thanks, Amy Lee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GPT2/ALT2 antibody
Hello histonet, Has anyone done GPT2/ALT2 IHC? Could you recommend an antibody works well on paraffin rat section? I searched around but don't know which one is good. Thank you, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] renin IHC
Hello, I am looking for a renin antibody for IHC on FFPE rodent tissue. I searched around and couldn't decide which one I should try. Could anybody recommend one? Thanks in advance. Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How to fix frozen section?
Hello, I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] monkey control slides
Hello, I was asked to do IHC on monkey atrium, kidney and spleen or liver tissue. Could anybody tell me which company carry these monkey slides? Thanks! Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] neutrophil antibody on rat FFP section
Hello, I was asked to perform neutrophil IHC on rat FFP sections. I searched around and found most are for mouse tissue. There is one from Serotec mouse anti rat but it does not work on paraffin section. Could anybody recommend a good antibody? Thanks in advance, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] keep deparaffinized and rehydrated slides in dH2O for a few days
Hi histonetters, Is it safe to keep deparaffinized and rehydrated slides in dH2O at 4 degree for 4 to 5 days before doing IHC? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thank you all sho gave me advice on reducing background of liver tissue!
___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC blocking for liver tissue
Hello, I have a question that what is best way to block endougenous biotin and HRP in liver or kidney tissue. The method I am using has to use avidin-biotin and HRP reactions. This is fine on heart tissue. I used biotin blocking kit from DAKO or Vector. Also I used 1% H2O2. But liver tissue still show background stain. Could you teach me a better way to block or is there other reason cause background? Thanks in advance, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] To detect injected mouse antibody inside mouse tissue
Hello, I have a question regarding detecting injected mouse antibody in mouse tissue by IHC. Now I have mouse liver paraffin section. This mouse was injected mouse antibody. I was asked to locate this antibody. This is a mouse chimeric antibody. Variable regions from human antibody was combined with mouse IgG2a heavy chain and kappa light chain constant regions. I am thinking using rabbit anti-human IgG, F(ab) fragment specific. Do you think it's doable or you have any good suggestion? When this mouse antibody bind to antigen inside liver through this human variable region, my F(ab) fragment specific antibody can still bind to it? Hope I describ my questions clearly. Thank you in advance for any help! Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] hyaluronidase for IHC pretreatment
Hello, I saw a protocol using hyaluronidase digestion for IHC pretreatment. But I couldn't find the way how to make this solution. Could anybody help me please? Best regards, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] table for microscope
Hello, I am looking for a microscope table. It doesn't has to be designed specific for microscope. The microscope I am using is normal sized Nikon E800. I have a computer sit besides. Do you know where I can look? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] collagen III antibody
Hello, I am looking for a collagen III antibody that works on FFPE mouse tissue. Could anybody recommand one please? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BrdU immunostain on rat brain section
Hello, I was asked to do BrdU immunostain on rat brain tissue. Does anyone have experience of it? 1.Do you prefer frozen or paraffin? 2.Which antibody you recommend? 3. If 35um frozen is requied, what thickness you will use if you want go for paraffin? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] staining for elastic fiber of artery in rat lung
Hello histonetters, Would you teach me what is best stain for elastic fiber in rat lung except weigert's stain? I want to look at arteries. Thanks in advance, Weihua ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
